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A cloud point extraction CPE procedure was developed for the separation and preconcentration of trace amounts of cobalt prior to its determination by flame atomic absorption spectrometry FAAS. The procedure is based on the formation of Co II complex with 2-[ 2-mercaptophenylimino methyl]phenol MPMP followed by its extraction into a Triton X surfactant-rich phase. Some important parameters affecting both complexation and extraction, such as pH, concentrations of chelating agent and surfactant, temperature, and time of equilibration, were optimized. Under optimum conditions, the preconcentration of a volume of 25 mL of sample resulted in a limit of detection of 0. This procedure was successfully applied to water, vitamin B12 and B-complex samples. The accuracy was assessed employing addition-recovery experiments, independent analysis by graphite furnace atomic absorption spectrometry and by using a certified reference material.
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Indirect flow-injection determination of ascorbic acid by flame atomic absorption spectrometry

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A continuous-flow procedure is proposed for the indirect determination of ascorbic acid, based on its reducing properties because of the oxidation of its 1,2-enediol group. Iron III was injected into a 1,phenanthroline stream, which was mixed with a sample carrier and then with a sodium picrate solution stream. The unadsorbed iron was determined by flame atomic absorption spectrometry. The proposed method allows the determination of ascorbic acid in the range 0. This method has been applied to the determination of ascorbic acid in pharmaceutical preparations, fruit juices and sweets. The results obtained in the analysis are compared with those provided by the 2,6-dichloroindophenol method. This is a preview of subscription content, access via your institution.
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Pages may load more slowly than usual and you may not be able to access some content or log in. If this happens then please refresh your web browser or try waiting two to three minutes before trying again. Sorry for any inconvenience and thank you for your patience. The concept of cloud point phenomenon is used to perform metal speciation after the studied metallic forms have reacted with suitable ligands for the formation of hydrophobic complexes, which are subsequently entrapped in the surfactant micelles. As an analytical demonstration, trace concentrations of Cr III and Cr VI were conveniently detected in samples with a complex matrix, such as sea water , without any laborious and expensive treatment using flame atomic absorption spectrometry.
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The behavior of surfactants of different natures and chain lengths was studied in flame atomic absorption spectrometry FAAS analyses. The variations of absorbance, which arise as a consequence of the surfactant addition to aqueous solutions of Cr VI or Cr III , were measured. Depressions were observed below the critical micelle concentration CMC , whereas enhancements were observed above the CMC. These depressions are more significant when the surfactant is opposite in charge to the analyte and the longest surfactant chain is used. A mechanism that enables explanation of the effects of a surfactant on FAAS is also suggested.
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